RBS

Part:BBa_K4733001:Design

Designed by: Diego Perez Hidalgo   Group: iGEM23_UBC-Okanagan   (2023-10-05)


B. Subtilis RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The UBC-Okanagan 2023 team used Modular Cloning (MoClo) standardization for this part: added Type II restriction sites and standard flanks to this part.


Source

This RBS sequence was sourced from several genes from the B. Subtilis genome. This study was done by Rocha et al. 1999.


References

Rocha, E. P. C., Danchin, A., & Viari, A. (1999). Translation in Bacillus subtilis: roles and trends of initiation and termination, insights from a genome analysis. Nucleic Acids Research, 27(17), 3567–3576. https://doi.org/10.1093/nar/27.17.3567